
Thermo Fisher Scientific SSEA5 Monoclonal Antibody (8E11)
인간 배아줄기세포에서 SSEA-5 항원을 인식하는 Mouse 모노클로날 항체. ICC/IF 및 유세포분석에 적합하며, 단백질 A로 정제된 1 mg/mL 액상 형태. 배양된 인간 배아암종 세포에서 양성 반응 확인.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:50–1:400 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8E11 |
| Immunogen | Undifferentiated H9 human embryonic stem cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536848 |
Additional Formats
Product Specific Information
Immunofluorescence and flow cytometric analysis of MA1-144 shows positive staining of SSEA-5 positive pluripotent cells within cultured human embryonal carcinoma NCCIT and NTERA-2 cells. Mouse teratocarcinoma cell line F9 was used as a negative control and showed no staining.
Target Information
Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-β1-3GlcNAc-β, characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells, and on the surface of induced pluripotent cells. SSEA-5 expression precedes the loss of other known stem cell markers, and its combination with CD9 and CD90 or CD50 and CD200 improves identification and elimination of rare undifferentiated human ES cells from differentiated populations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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