Thermo Fisher Scientific SSEA5 Monoclonal Antibody (8E11), DyLight 488
인간 SSEA-5 항원을 인식하는 DyLight 488 결합 단클론 항체로, 줄기세포 표지 연구에 적합합니다. ICC/IF 및 유세포분석에 사용 가능하며, 높은 특이성과 재현성을 제공합니다. 단백질 A 정제, 액상 형태로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:10–1:100 |
| Flow Cytometry (Flow) | 1:50–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8E11 |
| Immunogen | Undifferentiated H9 human embryonic stem cells |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536849 |
Additional Formats
Product Specific Information
Immunofluorescence and flow cytometric analysis of MA1-144-D488 shows positive staining of SSEA-5 positive pluripotent cells and cultured human embryonal carcinoma NCCIT cells.
Target Information
Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-β1-3GlcNAc-β, which is characteristic of H-1 antigens.
SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells, and on the surface of induced pluripotent cells.
SSEA-5 was found to precede the loss of other known stem cell markers. The combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations compared to the use of other established stem cell markers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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