Thermo Fisher Scientific SSEA5 Monoclonal Antibody (8E11)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:50-1:400

-

Flow Cytometry (Flow)

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8E11

Immunogen

Undifferentiated H9 human embryonic stem cells

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• DyLight 488
• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536848

Product Specific Information

Immunofluorescence and flow cytometric analysis of MA1-144 shows positive staining of SSEA-5 positive pluripotent cells within cultured human embryonal carcinoma NCCIT and NTERA-2 cells. Mouse teratocarcinoma cell line F9 was used as a negative control and showed no staining.

Target Information

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.