Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 555

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:25-1:100

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Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

L5

Immunogen

DYKDDDDK-tagged mouse Langerin

Conjugate

Alexa Fluor™ 555 Alexa Fluor™ 555 Alexa Fluor™ 555

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 647
• Request custom conjugation

Excitation/Emission Max

553/568 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610654

Product Specific Information

similar epitope as the Flag tag from Sigma

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.