Thermo Fisher Scientific Arginase 1 (ARG1) Monoclonal Antibody (OTI2E7)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme that catalyzes the conversion of L-arginine to urea and L-ornithine, the final step in the urea cycle. The resulting polyamines are essential for cell proliferation and detoxification of protein degradation byproducts.
By degrading arginine, Arginase 1 limits nitric oxide synthesis by depriving NO synthase of its substrate, thereby down-regulating NO production.

In both humans and mice, Arginase 1 is expressed in the liver, neutrophils, myeloid-derived suppressor cells (MDSCs), and neural stem cells.
In humans, it is expressed in blood neutrophils but not in CCR3+ granulocytes. In mice, Arginase 1 expression is a hallmark of alternatively activated macrophages (M2a).
It may also be expressed in tumor-infiltrating myeloid cells and is commonly detected in hepatocellular carcinoma.
Defects in the ARG1 gene cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.