Thermo Fisher Scientific NFKB1 Monoclonal Antibody (OTI10D7)

Applications

Western Blot (WB)

• Tested Dilution: 1:250–1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

NF-κB is a nuclear transcription factor activated by various extracellular and intracellular stimuli such as cytokines, UV radiation, stress, and microbial products.
It regulates key cellular processes including growth, differentiation, proliferation, apoptosis, and inflammation.
NFKB1 (p50) is a 50 kDa subunit of NF-κB, synthesized as a p105 precursor containing conserved domains for nuclear localization, DNA binding, and dimerization.
It forms homodimers or heterodimers (e.g., with p65) to generate functional NF-κB complexes.
NF-κB plays roles in immune response and has been implicated in AIDS, metastatic cancers, and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.