Thermo Fisher Scientific Ataxin 1 Monoclonal Antibody (N65/37), FITC
Ataxin 1 단백질을 검출하기 위한 FITC 결합 단클론 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 Immunocytochemistry에 사용 가능하며, 단백질 G로 정제된 액상 형태입니다. 4°C에서 암소 보관하며 연구용으로만 사용됩니다.
- 카탈로그번호
- MA545706
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N65/37 |
| Immunogen | Synthetic peptide amino acids 746-761 (RKRRWSAPETRKLEKS) of mouse ataxin-1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932160 |
Additional Formats
- Unconjugated (MA5-27667)
- APC (MA5-45705)
- PE (MA5-45708)
- PerCP (MA5-45707)
- Custom conjugation available upon request
Product Specific Information
- Rat: 93% identity (15/16 amino acids identical)
- Human: 87% identity (14/16 amino acids identical)
- 1 µg/mL of MA5-45706 was sufficient for detection of Ataxin 1 in 20 µg of COS cells transiently transfected with Ataxin-1 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 85 kDa.
- No cross-reactivity against phospho S751-Ataxin-1.
- Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence; negative by immunoblot.
- Formerly sold as clone S65-37.
Target Information
The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem, and spinal cord. ADCA is divided into three groups (types I–III). ADCAI is genetically heterogeneous with five loci (SCA1, SCA2, SCA3, SCA4, SCA6) mapped to different chromosomes. ADCAII (SCA7) presents with retinal degeneration, while ADCAIII (SCA5) is a “pure” cerebellar syndrome.
ADCA results from CAG repeat expansion producing elongated polyglutamine tracts in the ataxin proteins. The diseased allele contains 41–81 CAG repeats compared to 6–39 in normal alleles, associated with spinocerebellar ataxia type 1 (SCA1). The function of ataxins remains unclear. This locus maps to chromosome 6, with at least two transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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