Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H4), FITC

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC (Cat. No. MA5-45709)
• PerCP (Cat. No. MA5-45711)
• Custom conjugation available upon request

Product Specific Information

1 µg/mL of MA5-45710 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The transcription of heat shock genes is regulated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs).
HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and are conserved among species. Although HSFs from yeast, Drosophila, and human differ in molecular weight and immunological reactivity, two HSFs have been identified in human cells: HSF1 and HSF2.
HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 is activated to a nuclear-localized, trimeric form that binds DNA, and its phosphorylation is required for maximal transcription of heat shock genes.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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