Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), Alexa Fluor 488, eBioscience
GFAP 단백질을 인식하는 GA5 단클론 항체로, Alexa Fluor 488 형광 표지. 다양한 종(인간, 마우스, 랫트 등)에 반응. IHC, ICC, Flow Cytometry 등 다양한 응용 가능. 488 nm/519 nm 형광 특성, 4°C 암소 보관.
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Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 publications |
| Immunohistochemistry (IHC) | - | 37 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | 2 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 8 publications |
| Flow Cytometry (Flow) | Assay-Dependent | 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rabbit, Rat |
| Published Species | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GA5 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597754 |
Additional Formats
- Unconjugated
- eFluor 570
- eFluor 615
- eFluor 660
- Custom conjugation available
Product Specific Information
This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants exist, with alpha-GFAP being the predominant form in astrocytes.
GFAP can co-assemble with vimentin and nestin, and its assembly depends on phosphorylation and dephosphorylation of the N-terminal domain. Mutations in the GFAP gene are associated with Alexander disease, and GFAP is often overexpressed in glial-derived tumors.
Applications Reported:
Intracellular staining with flow cytometry, immunohistology of frozen and FFPE tissue sections, and immunocytochemistry.
Applications Tested:
Validated in immunocytochemistry (C6 cells) and immunohistochemistry (FFPE human tissue, low pH antigen retrieval). Recommended ≤10 µg/mL; titration advised for optimal results.
Fluorescence Characteristics:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein found predominantly in astrocytes and certain glial cells of the CNS, as well as in peripheral ganglia and Schwann cells. It serves as a key marker for astrocytic cells and is expressed in neural stem cells. GFAP expression is also observed in various brain tumors, lens epithelium, Kupffer cells, and some salivary tumors. Mutations in GFAP cause Alexander disease. Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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