Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• eFluor 570
• eFluor 615
• eFluor 660
• Custom conjugation available

Product Specific Information

This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants exist, with alpha-GFAP being the predominant form in astrocytes.

GFAP can co-assemble with vimentin and nestin, and its assembly depends on phosphorylation and dephosphorylation of the N-terminal domain. Mutations in the GFAP gene are associated with Alexander disease, and GFAP is often overexpressed in glial-derived tumors.

Applications Reported:
Intracellular staining with flow cytometry, immunohistology of frozen and FFPE tissue sections, and immunocytochemistry.

Applications Tested:
Validated in immunocytochemistry (C6 cells) and immunohistochemistry (FFPE human tissue, low pH antigen retrieval). Recommended ≤10 µg/mL; titration advised for optimal results.

Fluorescence Characteristics:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein found predominantly in astrocytes and certain glial cells of the CNS, as well as in peripheral ganglia and Schwann cells. It serves as a key marker for astrocytic cells and is expressed in neural stem cells. GFAP expression is also observed in various brain tumors, lens epithelium, Kupffer cells, and some salivary tumors. Mutations in GFAP cause Alexander disease. Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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