Thermo Fisher Scientific Myosin 4 Monoclonal Antibody (MF20), eBioscience
MF20 단일클론 항체로 골격근 및 심근의 Myosin II 중 무거운 사슬을 인식합니다. Western blot과 면역세포화학(ICC/IF)에 최적화되어 있으며 다양한 척추동물 종에 반응합니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Thermo Fisher Scientific Myosin 4 Monoclonal Antibody (MF20), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | 8 |
| Immunohistochemistry (IHC) | – | 7 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 2 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | 15 |
| Flow Cytometry (Flow) | – | 3 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Chimpanzee, Cat, Guinea pig, Human, Mouse, Non-human primate, Rabbit, Rat |
| Published Species | Chicken, Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MF20 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865201 |
Additional Formats
Product Specific Information
This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specifically the light meromyosin portion, in cardiac and skeletal muscle of vertebrates.
Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing.
Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.
MF20 reacts with myosin from various mammalian, avian, and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.
Applications Reported: Western blotting and immunocytochemistry.
Applications Tested: Tested by ICC and WB; recommended ≤1 µg/mL.
Careful titration is advised for optimal assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The target protein is a fast myosin isoform expressed in skeletal muscle, involved in muscle contraction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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