Thermo Fisher Scientific Neural/Glial Antigen 2 (NG2) Monoclonal Antibody (9.2.27), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Alexa Fluor 488
• Request custom conjugation

Product Specific Information

This 9.2.27 monoclonal antibody reacts with human neural/glial antigen 2 (NG2), also known as melanoma chondroitin sulfate proteoglycan (MCSP). The antigen consists of a 250 kDa N-linked glycoprotein and a >450 kDa proteoglycan. NG2 is expressed on more than 90% of malignant melanomas and certain non-melanomic tumors, as well as glioma cells and oligodendrocyte precursor cells in both developing and adult tissues.

NG2 is involved in tumor cell proliferation, adhesion, migration, and invasion, and functions as a coreceptor for α4β1 integrin. Expression of NG2 can serve as a prognostic marker for disorders such as acral lentiginous melanoma and infantile acute myeloid leukemia. The 9.2.27 antibody has been reported to suppress melanoma tumor growth.

Applications Reported: Flow cytometric analysis, immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue (IHC-P), and immunocytochemical staining of fixed cells (ICC).

Applications Tested:

• Tested by ICC on methanol-fixed A375 cells ≤10 µg/mL
• Tested by Flow cytometry on A375 cells ≤0.125 µg/test
  A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). Titrate for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CSPG4 (chondroitin sulfate proteoglycan 4) is a human melanoma-associated surface proteoglycan involved in stabilizing cell-substratum interactions during melanoma cell spreading on endothelial basement membranes. It is an integral membrane protein expressed by human malignant melanoma cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.