Thermo Fisher Scientific Oligodendrocyte Marker O1 Monoclonal Antibody (O1), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

O1

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865205

Product Specific Information

Description: This O1 monoclonal antibody reacts with galactocerebroside and other lipids found on late oligodendrocyte precursors. O1 has been reported to react to all species tested (rat, mouse, human, chicken). Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.

Applications Reported: This O1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, ELISA, and immunocytochemistry.

Applications Tested: This O1 antibody has been tested by immunocytochemistry on formaldehyde-fixed, differentiated OLN93 cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.