Thermo Fisher Scientific alpha-Fetoprotein Monoclonal Antibody (AFP3), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

-

Immunocytochemistry (ICC/IF)

1-10 µg/mL

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Flow Cytometry (Flow)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

AFP3

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865213

Product Specific Information

Description: This AFP3 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions.

When performing western blotting or immunohistochemistry on paraffin section, we recommend the use of monoclonal antibody 1E8 (Product # 14-9499).

Applications Reported: This AFP3 antibody has been reported for use in immunoblotting (WB) and immunocytochemistry.

Applications Tested: This AFP3 antibody has been tested by western blot analysis of reduced HepG2 cell lysate, as well as by immunocytochemistry of HepG2 cells. For western blotting, this antibody can be used at 1-10 µg/mL. (Western blotting of non-reduced cell lysate yields a band at approximately 58 kDa.) For immunocytochemistry, AFP3 can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.