Thermo Fisher Scientific FOXP3 Monoclonal Antibody (SP97)

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
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Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100
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Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer (pH 8.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, essential for immune homeostasis.
It is 381 amino acids long and is highly expressed in CD25⁺ CD4⁺ regulatory T cells, low in CD4⁺/CD25⁻ cells, and absent in CD4⁻/CD8⁺ T cells.
FOXP3 acts as a master regulatory gene and a specific marker of regulatory T cells.
Mutations in FOXP3 cause the scurfy phenotype in mice and are associated with IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome) in humans.
Expression of FOXP3 in human breast and colon cancer cells is regulated by p53 in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.