Thermo Fisher Scientific Glypican 3 Monoclonal Antibody (SP86)
Glypican 3 단백질을 인식하는 Rabbit Monoclonal Antibody (SP86). Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. Protein A 정제, PBS/BSA buffer에 보관. 인체 시료 반응성 검증 완료. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| Flow Cytometry (Flow) | 1:80 | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP86 |
| Immunogen | Synthetic peptide derived from C-terminus of human glypican-3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.466 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537887 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended prior to staining using 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
- After retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
- Suggested positive control: placenta or liver carcinoma.
Target Information
GPC3 (Glypican 3) is a cell surface proteoglycan bearing heparan sulfate. It may function in the suppression or modulation of growth in mesodermal tissues and organs and influence IGF2 receptor interactions.
Members of the glypican family are GPI-anchored membrane proteoglycans involved in cell division, growth regulation, and tumor predisposition.
Mutations in GPC3 cause Simpson-Golabi-Behmel syndrome (SGBS), characterized by pre- and postnatal overgrowth with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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