Thermo Fisher Scientific Cytokeratin 14 Monoclonal Antibody (SP53)
Cytokeratin 14 단백질을 인식하는 Rabbit Monoclonal Antibody(SP53)로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성이 있으며, Protein A/G 정제 및 PBS buffer에 보관됩니다.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:25 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1:100–1:1,900 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP53 |
| Immunogen | Synthetic peptide corresponding to the C-terminus of human keratin 14 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537889 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
- After retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
- Suggested positive controls: skin, squamous cell carcinoma, or prostate tissue.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, found in both keratinizing tissues (e.g., skin) and non-keratinizing cells (e.g., mesothelial cells). At least 20 different CKs have been identified and are classified into two main types:
- Type I (acidic polypeptides)
- Type II (basic polypeptides)
Keratin 14 belongs to the type I subfamily of low molecular weight keratins and typically pairs with keratin 5. It helps distinguish stratified epithelial cells from simple epithelial cells and is useful in identifying squamous cell carcinomas. Keratin 14 is also considered a prognostic marker in breast carcinomas. Both acidic and basic keratin family members are expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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