Thermo Fisher Scientific Cytokeratin 14 Monoclonal Antibody (SP53)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:500

-

Immunocytochemistry (ICC/IF)

5µg/mL

-

Flow Cytometry (Flow)

1:100-1:1,900

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP53

Immunogen

Synthetic peptide corresponding to C-terminus of human keratin 14 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 14,uniProtId:P02533-1,ncbiNodeId:9606,antigenRange:472,antigenLength:472,antigenImageFileName:MA5-16370_Cytokeratin_14_P02533-1_Rabbit.svg,antigenImageFileNamePDP:MA5-16370_Cytokeratin_14_P02533-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537889

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, squamous cell carcinoma or prostate tissue.

Target Information

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.