Thermo Fisher Scientific CD172a (SIRP alpha) Monoclonal Antibody (15-414), Super Bright 436, eBioscience

Thermo Fisher Scientific CD172a (SIRP alpha) Monoclonal Antibody (15-414), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 1 publication

Product Specifications

Additional Available Formats

• APC
• FITC
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

This 15-414 monoclonal antibody reacts with human CD172a (SIRP alpha), a cell surface glycoprotein with three Ig-like extracellular domains and two cytoplasmic ITIM motifs. These motifs recruit SHP-1 and SHP-2 phosphatases. CD172a is expressed on monocytes, granulocytes, dendritic cells, and CD34⁺CD38⁻ progenitor cells, but not on T or B lymphocytes. Neurons also express CD172a. Its ligand, CD47, mediates dendritic cell–T cell activation, neutrophil migration, and phagocytosis.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
• Recommended Use: 5 µL (0.5 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)

Fluorochrome Information:
Super Bright 436 is excited by a 405 nm violet laser and emits at 436 nm. Use a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.

Buffer Recommendation:
For panels using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

SIRP alpha (CD172a) is a receptor-type transmembrane glycoprotein expressed on myeloid cells (granulocytes, DCs, macrophages, mast cells, hematopoietic stem cells). It acts as a substrate for tyrosine kinases (EGFR, PDGFR, src, insulin receptor) and negatively regulates receptor tyrosine kinase signaling. CD47 binding triggers phosphorylation of ITIMs, recruiting SHP-1 and SHP-2 phosphatases. This interaction regulates macrophage phagocytosis and T cell/DC activation. SIRP alpha influences cellular responses to serum, growth factors, insulin, oncogenes, and adhesion. Cancer cells overexpress CD47, activating SIRP alpha to inhibit macrophage-mediated tumor cell destruction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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