Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), Super Bright 436, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 2 publications

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• View 13 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by subsets of tissue macrophages such as splenic red pulp macrophages, Kupffer cells, and intestinal lamina propria macrophages. Mouse monocytes do not express CD163, and it is not a reliable M2 macrophage marker in mice.

This antibody detects CD163 on fixed and permeabilized cells, allowing intracellular staining. For tissues subjected to aggressive dissociation, intracellular detection is recommended.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤0.25 µg/test)
• Excitation: 405 nm
• Emission: 436 nm
• Recommended Filter: 450/50 bandpass

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily. It acts as a receptor for the hemoglobin-haptoglobin complex, protecting tissues from oxidative damage and participating in iron recycling.

CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and can be shed from the cell surface upon stimulation with LPS or PMA. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner and triggers intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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