Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Super Bright 436, eBioscience

Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Super Bright 436, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 660
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 800
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 690
• NovaFluor Yellow 810
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

This 1RNM44N monoclonal antibody recognizes mouse CD170 (Siglec F), a cell surface lectin of the Ig superfamily that binds sialic acid-containing glycoconjugates.
Siglec F is mainly expressed on eosinophils and alveolar macrophages, with lower levels on immature myelomonocytic cells. It serves as a functional ortholog of human Siglec 8 but is not expressed on mast cells.

• Recognizes a formaldehyde-fixed epitope
• Reported for use in flow cytometric analysis
• Tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages
• Recommended use ≤ 0.125 µg/test (100 µL final volume)
• Cell number range: 10⁵–10⁸ cells/test
• Optimal titration recommended for best assay performance

Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Use a 450/50 bandpass filter or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

SIGLEC5 is a putative adhesion molecule mediating sialic-acid dependent binding to cells, binding both α2,3- and α2,6-linked sialic acids.
Expression is high in peripheral blood leukocytes, spleen, and bone marrow, and lower in lymph node, lung, appendix, placenta, pancreas, and thymus.
Expressed by monocytes and neutrophils, but absent from leukemic cell lines representing early myelomonocytic differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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