Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), PE-Cyanine7, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Flow Cytometry (Flow)

1.0 µg/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1D4B (1D4B)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

View additional formats

• Unconjugated
• Alexa Fluor 488
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• PE
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848304

Product Specific Information

Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders.

Applications Reported: This eBio1D4B antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio1D4B antibody has been tested by by intracellular staining followed by flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer ((Product # 00-8222-49)) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution ((Product # 00-5333-57)) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer`s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.