Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), PE-Cyanine7, eBioscience
마우스 CD107a(LAMP-1)를 인식하는 eBio1D4B 단클론 항체로, PE-Cyanine7 형광체가 결합된 제품입니다. Flow Cytometry용으로 최적화되어 있으며, 세포 내 염색에 적합합니다. 광민감성이 있으므로 암조건에서 보관해야 합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 1.0 µg/test | View 12 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D4B (1D4B) |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848304 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- PE
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBio1D4B monoclonal antibody reacts with mouse CD107a (LAMP-1), a type I lysosomal membrane protein that is extensively glycosylated. CD107a is constitutively expressed in late endosomes-lysosomes and transiently on the surface of degranulating cytolytic T cells. It is involved in cancer metastasis and lysosomal storage disorders.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤1.0 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Optimize antibody concentration for best results.
Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57).
Excitation / Emission / Laser: 488–561 nm / 775 nm / Blue, Green, Yellow-Green Laser.
Target Information
LAMP1 (CD107a) is a major lysosomal membrane component, with 1–2% found on the plasma membrane. It is a heavily glycosylated type I integral membrane protein involved in lysosome biogenesis and fusion with phagosomes. Upon activation, LAMP1 translocates to the plasma membrane via a tyrosine-based motif (YXXI). Surface LAMP1 promotes PBMC adhesion to vascular endothelium, suggesting a role in inflammation. Increased LAMP1 is observed near senile plaques in Alzheimer’s Disease and serves as a marker for mast cell activation.
For Research Use Only. Not for use in diagnostic procedures.
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