Thermo Fisher Scientific c-Myc Tag Monoclonal Antibody (2G8D5), THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.05-0.5 µg/mL

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Immunoprecipitation (IP)

1 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 22 publications 22 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2G8D5

Immunogen

A synthetic peptide EQKLISEEDL coupled to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• HRP
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.

The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Target Information

Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.