Thermo Fisher Scientific DYNC1I2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human DYNC1I2, validated for WB, IHC, and IP applications. Recognizes residues 1–50 of cytoplasmic dynein intermediate chain 2. Supplied as liquid, 1 mg/mL, stored at 4°C. Predicted to cross-react with bovine and or...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 to 50 of human Dynein, Cytoplasmic 1, Intermediate Chain 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with bovine and orangutan
Target Information
Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into mechanical energy of movement. Cytoplasmic and axonemal dynein complexes are minus end-directed motors responsible for intracellular cargo transport and ciliary motion.
Cytoplasmic dynein consists of approximately 12 subunits, including two heavy chains, two intermediate chains for cargo anchoring, and several smaller intermediate and light chains. It plays essential roles in organelle transport and centrosome assembly. The carboxy-terminus of dynein is conserved for motility, while the amino-terminal regions vary. Dynein activity is regulated by associated proteins such as Dynactin, LIS1, and Nudel (nudE-like).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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