Thermo Fisher Scientific Huntingtin Monoclonal Antibody (1HU-4C8)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:200

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

MA3-040 has been used successfully in Western blotting to detect huntingtin in brain lysates from mouse and rat.

Target Information

Huntingtin is a disease gene linked to Huntington’s disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, translating as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10–20 years.

The huntingtin locus spans 180 kb and consists of 67 exons. It is widely expressed and required for normal development. Two alternatively polyadenylated transcripts (~13.7 kb and ~10.3 kb) show different abundance in fetal and adult tissues. The larger transcript is mainly expressed in brain, while the smaller transcript is more widely distributed. The genetic defect may confer a new property on the mRNA or alter protein function rather than eliminating transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.