Thermo Fisher Scientific CHRNA1 Monoclonal Antibody (88B)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA3-043 detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in Torpedo and the delta subunit in mouse, human, rat, chicken, and frog.
This antibody does not detect the alpha 1 and beta 1 subunits.

MA3-043 has been successfully used in Western blot, immunohistochemistry, and immunoprecipitation procedures.
By Western blot, this antibody detects:

• A 60 kDa protein representing the gamma subunit
• A 65 kDa protein representing the delta subunit from Torpedo skeletal muscle homogenates

Under non-reducing conditions, the delta subunit migrates mostly as a dimer of ~130 kDa.
Immunohistochemical staining of nAChR in rat skeletal muscle with MA3-043 results in strong staining of the motor endplate.

In immunohistochemical staining, cryostat sectioning or permeabilization is recommended due to the antibody’s reactivity with the cytoplasmic side of the receptor.

The MA3-043 antigen is purified Torpedo californica acetylcholine receptor.

Target Information

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction.
The channel-linked AChR, mediating rapid excitatory actions of acetylcholine, is called nicotinic AChR (nAChR) because it can be activated by nicotine.
The non-channel linked AChR, mediating slower actions of acetylcholine (inhibitory or excitatory), is called muscarinic AChR (mAChR) because it can be activated by muscarine.

nAChRs are classified into two subclasses:

• Muscle-type receptor: Pentameric glycoprotein composed of five membrane-spanning subunits (2 α1, β1, γ or ε, and δ), forming a ligand-gated ion channel.
• Neuronal-type receptor: Also a pentamer, composed of α and β subunits (α2–α8 and β2–β4).

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.