Thermo Fisher Scientific Acetylcholinesterase Monoclonal Antibody (HR2)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Cat, Guinea pig, Human, Mouse, Rabbit

Published species

Guinea pig, Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

HR2

Immunogen

Purified human cerebellar acetylcholinesterase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Acetylcholinesterase,uniProtId:P22303-1,ncbiNodeId:9606,antigenRange:1-614,antigenLength:614,antigenImageFileName:MA3-042_Acetylcholinesterase_P22303-1_House_mouse.svg,antigenImageFileNamePDP:MA3-042_Acetylcholinesterase_P22303-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325478

Product Specific Information

MA3-042 detects acetylcholinesterase (AChE) from human, mouse, rabbit, guinea pig, bovine and cat tissues. This antibody does not cross react with rat or frog AChE, and does not detect butyrylcholinesterase (BChE).

MA3-042 has been successfully used in immunohistochemistry, immunoprecipitation and ELISA procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of AChE in human brain samples with MA3-042 results in staining of nerve fibers and terminals.

The MA3-042 antigen is purified human cerebellar acetylcholinesterase.

Target Information

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.