Thermo Fisher Scientific SMAD1 Monoclonal Antibody (1356CT119.18.55)
SMAD1 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. 단백질 G로 정제된 0.5 mg/mL 액상 형태. 인간 샘플에 반응하며, BMP 신호전달 연구에 적합. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:25 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1356CT119.18.55 |
| Immunogen | Recombinant protein between 20–330 amino acids from human SMAD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897662 |
Target Information
The protein encoded by this gene belongs to the SMAD family, which shares similarity with the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins act as signal transducers and transcriptional modulators that mediate multiple signaling pathways.
This protein mediates bone morphogenetic protein (BMP) signaling, which regulates cell growth, apoptosis, morphogenesis, development, and immune responses. Upon BMP ligand stimulation, SMAD1 is phosphorylated by BMP receptor kinases and forms a complex with SMAD4 to regulate transcription.
SMAD1 is a target for SMAD-specific E3 ubiquitin ligases (e.g., SMURF1, SMURF2) and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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