Thermo Fisher Scientific HIF1AN Monoclonal Antibody (931CT3.1.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

Immunocytochemistry (ICC/IF)

1:25

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

931CT3.1.1

Immunogen

KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF1AN,uniProtId:Q9NWT6-1,ncbiNodeId:9606,antigenRange:1-349,antigenLength:349,antigenImageFileName:MA5-37749_HIF1AN_Q9NWT6-1_House_mouse.svg,antigenImageFileNamePDP:MA5-37749_HIF1AN_Q9NWT6-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.45 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897673

Target Information

Hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.