Thermo Fisher Scientific IGF2R Monoclonal Antibody (2G11)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA1-066 detects mannose 6-phosphate receptor (MPR) from human, rat, monkey, and bovine tissues. This antibody does not detect Chinese hamster ovary cell MPR.
It has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry, and immunoprecipitation procedures.
By Western blot, this antibody detects a ~300 kDa protein representing MPR in HeLa cell extract under non-reducing conditions.
Immunofluorescence staining of MPR in HeLa cells with MA1-066 results in perinuclear staining.
The immunogen is purified bovine ~300 kDa CI-MPR, and the antibody recognizes an epitope in the extracellular domain of MPR.

Target Information

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are transported within the cell by approximately 300 kDa cation-independent mannose 6-phosphate receptors (CI-MPR).
MPRs cycle between the trans-Golgi network, endosomes, and the cell surface every 3 hours.
The steady-state distribution of MPRs is mainly in late endosomes, fewer in the trans-Golgi network, and about 10% at the cell surface.
CI-MPR binds both man6P and IGF-II simultaneously and also exists as a soluble truncated form (~240 kDa) circulating in serum, capable of ligand binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.