Thermo Fisher Scientific SPANX-C Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (IHC)

1.25-2.5 µg/mL

-

ELISA (ELISA)

1:5,000-1:20,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANX-C protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPANXC,uniProtId:Q9NY87-1,ncbiNodeId:9606,antigenRange:1-97,antigenLength:97,antigenImageFileName:200-401-915_SPANXC_Q9NY87-1_Rabbit.svg,antigenImageFileNamePDP:200-401-915_SPANXC_Q9NY87-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

5 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This Protein A purified antibody is directed against human SPANX-C protein. A protein-protein BLAST analysis was used to suggest cross reactivity with most SPANX proteins from human, gorilla and chimpanzee. Reactivity against homologues from is not known.

Target Information

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a protein that localizes to the nucleus and is expressed in highly metastatic cell lines, making the protein a potential diagnostic and prognostic marker. The protein belongs to a family of cancer/testis antigens and represents a potential target for cancer immunotherapy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.