Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• eFluor 450
• FITC
• PE
• Super Bright 600
• Custom conjugation available upon request

Product Specific Information

The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily characterized by four hydrophobic domains and short cytoplasmic termini. CD9 is expressed in various cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts, and neuroblasts.

On T cells, CD9 acts as a co-stimulatory molecule. CD9 deficiency in oocytes causes sterility due to defective gamete fusion. In macrophages, CD9 associates with FcγRs to modulate phagocytosis and inflammatory signaling. In B cells, CD9 serves as a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, CD9 facilitates MHC II molecule association. Expression levels may vary between donors.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells; ≤0.5 µg/test recommended. One test defines the amount of antibody to stain a cell sample in 100 µL volume. Cell number should be empirically determined (10⁵–10⁸ cells/test).
Fluorochrome details: Alexa Fluor 700 emits at 723 nm, excited by red laser (633–647 nm). Recommended filters: 685 LP mirror and 710/20 band pass filter.
Excitation/Emission: 633–647 nm / 723 nm; Laser: Red.

Target Information

CD9 antigen is a glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells. It belongs to the tetraspanin family, spanning the membrane four times. In pre-B cells and platelets, CD9 regulates activation and aggregation, possibly through integrin CD41/CD61 (GPIIb/GPIIIa) interaction.

CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion. It plays roles in cell attachment, proliferation, and association of MHC II molecules on dendritic cells, crucial for T cell stimulation. CD9 contributes to differentiation, adhesion, and signal transduction, and acts as a metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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