Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660
• APC, APC-eFluor 780
• Biotin, Brilliant UV/Violet series
• eFluor 450/506/615/660
• FITC, PE, PE-Cy5, PE-Cy5.5, PE-Cy7
• PerCP-Cy5.5, PerCP-eFluor 710
• Super Bright, NovaFluor series
• Functional Grade
• Custom conjugation available upon request

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as a heterodimer (CD8αβ) or homodimer (CD8αα).

• CD8αβ: expressed on most thymocytes and a subset of mature αβ TCR T cells
• CD8αα: expressed on γδ TCR T cells, intestinal intraepithelial lymphocytes, and dendritic cells

CD8 binds to MHC class I and interacts with tyrosine kinase p56lck, contributing to T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of mouse thymocyte and splenocyte suspensions (≤0.125 µg/test; 100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test).
Fluorochrome Properties:

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red laser
• Recommended filter: 685 LP mirror, 710/20 filter

Filtered post-manufacture (0.2 µm).

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein receptor of the immunoglobulin family.

• Functions as a co-receptor for MHC class I molecules
• Expressed mainly on cytotoxic T cells as CD8αβ heterodimers
• Also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8αα homodimers
• Engagement with MHC-I/peptide complexes recruits Lck kinase, inducing lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation
• CTLs are critical for pathogen and tumor cell clearance
• Differentiation of naïve CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1

For Research Use Only. Not for use in diagnostic procedures.

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