Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 700, eBioscience
Mouse CD8a 단백질을 인식하는 53-6.7 클론 단일클론 항체로, Alexa Fluor 700으로 표지됨. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.125 µg/test로 사용 가능. Affinity chromatography로 정제되었으며, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 20 |
| Immunocytochemistry (ICC/IF) | - | 6 |
| Flow Cytometry (Flow) | 0.125 µg/test | 201 |
| Immunoprecipitation (IP) | - | 1 |
| Functional Assay | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_494006 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660
- APC, APC-eFluor 780
- Biotin, Brilliant UV/Violet series
- eFluor 450/506/615/660
- FITC, PE, PE-Cy5, PE-Cy5.5, PE-Cy7
- PerCP-Cy5.5, PerCP-eFluor 710
- Super Bright, NovaFluor series
- Functional Grade
- Custom conjugation available upon request
Product Specific Information
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as a heterodimer (CD8αβ) or homodimer (CD8αα).
- CD8αβ: expressed on most thymocytes and a subset of mature αβ TCR T cells
- CD8αα: expressed on γδ TCR T cells, intestinal intraepithelial lymphocytes, and dendritic cells
CD8 binds to MHC class I and interacts with tyrosine kinase p56lck, contributing to T cell development and activation.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of mouse thymocyte and splenocyte suspensions (≤0.125 µg/test; 100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test).
Fluorochrome Properties:
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red laser
- Recommended filter: 685 LP mirror, 710/20 filter
Filtered post-manufacture (0.2 µm).
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein receptor of the immunoglobulin family.
- Functions as a co-receptor for MHC class I molecules
- Expressed mainly on cytotoxic T cells as CD8αβ heterodimers
- Also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8αα homodimers
- Engagement with MHC-I/peptide complexes recruits Lck kinase, inducing lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation
- CTLs are critical for pathogen and tumor cell clearance
- Differentiation of naïve CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1
For Research Use Only. Not for use in diagnostic procedures.
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