Thermo Fisher Scientific ATG4D Polyclonal Antibody
Rabbit polyclonal antibody targeting human ATG4D, validated for WB and ICC/IF. Recognizes ~53 kDa protein. Supplied as liquid, 1 mg/mL, for research use only. Suitable for autophagy-related protein detection.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from the N-terminal of Human ATG4D (aa 98–108) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735500 |
Product Specific Information
A 1:1,000 dilution of PA5-77781 was sufficient for detection of ATG4D in 15 µg of Human HeLa Cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. Detects approximately 53 kDa.
Target Information
Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein that localizes to the cytoplasm and belongs to the C-54 family of cysteine proteases. Expressed predominately in skeletal muscle, but also present in testis, Atg4D functions as a cysteine protease required for autophagy. It specifically cleaves the C-terminal region of target proteins, allowing them to bind to autophagosomes. The enzymatic activity of Atg4D is inhibited by N-ethylmaleimide, a thiol-reactive compound that modifies cystine residues in proteins and peptides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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