Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CEW33D), NovaFluor Red 710, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Dilution: 5 µL (0.8 µg)/test

Product Specifications

Available Conjugate Formats

• APC
• Biotin
• Brilliant UV 615
• eFluor 660
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• Custom conjugation available

Product Specific Information

The CEW33D monoclonal antibody reacts with human CD183 (CXCR3), a G protein-coupled chemokine receptor that binds CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC). CD183 is associated with type 1 immunity and is expressed on activated T cells, Th1 cells, CD8 effector cells, NK cells, NKT cells, and some B cells and plasmacytoid dendritic cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for human peripheral blood cells at 5 µL (0.8 µg) per test (100 µL final volume).
Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, featuring engineered fluorescent signatures optimized for minimal spectral spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

The CD183 (CXCR3) gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily and mediate leukocyte trafficking, integrin activation, cytoskeletal reorganization, and chemotaxis. Expression is prominent in effector/memory T cells and inflamed tissues. The receptor is a potential target for small-molecule antagonists in inflammatory disease research. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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