Invitrogen™

HCS LIVE/DEAD™ Green Kit

The HCS LIVE/DEAD™ Green Kit measures cytotoxicity by high content analysis with the Image-iT™ DEAD Green™ viability stain—a non-fluorescent and자세히 알아보기

The HCS LIVE/DEAD™ Green Kit measures cytotoxicity by high content analysis with the Image-iT™ DEAD Green™ viability stain—a non-fluorescent and cell impermeant compound that exhibits a strong fluorescent enhancement upon binding to DNA. Drugs and test compounds leading to serious cell injuries, including plasma membrane permeability, allow entry of the Image-iT™ DEAD Green™ viability stain. This two-color fluorescence assay also employs the use of a cell permeant nuclear segmentation tool that stains both live and dead cells, reflecting the total cell number within the sample. For total cell staining, the kit includes both a blue-fluorescent Hoechst 33342 and a near infrared-fluorescent, HCS NuclearMask™ Deep Red reagent. Either of these segmentation tools may be used with Image-iT™ DEAD Green™ viability stain for excellent discrimination of live and dead cells that survives fixation and permeabilization for combination with additional parameters of cytotoxicity or other biomarkers. The kit contains sufficient reagents for performing 2, 96-well plates.

사양

세포 유형Eukaryotic Cells

색상Green, Far-Red, Blue

검출 방법Fluorescence

용도(애플리케이션)Viability Assay

용도 (장비)Fluorescence Microscope, High Content Analysis Instrument

제품 유형HCS LIVE/DEAD Green Kit

염료 유형HCS NuclearMask™ Deep Red stain, Image-iT™ DEAD Green viability stain, Hoechst 33342

방출Visible, UV, near IR

형식96-well plate

제품라인LIVE/DEAD™, Molecular Probes™

수량1 kit

배송 조건Room Temperature

Unit SizeEach