Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CEW33D), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CEW33D), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

• APC
• Biotin
• Brilliant UV 615
• eFluor 660
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• Custom conjugation available

Product Specific Information

The CEW33D monoclonal antibody reacts with human CD183 (CXCR3), a G protein-coupled chemokine receptor that interacts with CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC). CD183 is associated with type 1 immunity and is expressed in Th1, CD8 effector, NK, and NKT cells, as well as some B cells and plasmacytoid dendritic cells. CD183-ligand interactions mediate infiltration of inflamed tissues in normal and autoimmune responses.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometry using normal human peripheral blood cells
  • Use 5 µL (0.8 µg) per test (100 µL final volume)
  • Suitable for 10⁵–10⁸ cells/test

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) improves staining performance and should be used in all NovaFluor experiments.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

The CD183 (CXCR3) gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These ligands belong to the CXC chemokine subfamily and mediate leukocyte trafficking via integrin activation, cytoskeletal rearrangement, and chemotaxis. CD183 expression is prominent in effector/memory T cells and inflamed tissues. It plays a key role in inflammatory cell recruitment and is a potential target for small-molecule antagonists in inflammatory disease therapies. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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