Thermo Fisher Scientific Phospho-PLD2 (Tyr511) Polyclonal Antibody
PLD2 Tyr511 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 사용 가능. 인간, 마우스, 랫트 반응성. 액상형, 1 mg/mL 농도, PBS/glycerol buffer. 내인성 phosphorylated PLD2 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PLD2 (Accession O14939), corresponding to amino acid residues around phosphorylated Tyr511. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816805 |
Product Specific Information
Antibody detects endogenous levels of PLD2 only when phosphorylated at Tyr511.
Target Information
PLD2 is a peripheral membrane protein belonging to the phospholipase D family, containing a PH domain, two PLD phosphodiesterase domains, and a PX (phox homology) domain that plays a regulatory role.
It catalyzes the hydrolysis of phosphatidylcholine (PC) to produce phosphatidic acid and choline.
PLD2 is implicated in regulated secretion, cytoskeletal regulation, endocytosis, transcriptional regulation, and cell cycle control.
It is activated by phosphatidylinositol 4,5-bisphosphate (PIP2) and ADP-ribosylation factor-1 (ARF-1), and interacts with EGFR and PIP5K1A.
Localized to plasma membrane caveolae, PLD2 is ubiquitously expressed in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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