Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• eFluor 450
• Functional Grade
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Custom conjugation available

Product Specific Information

The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184), also known as Fusin, LESTR, or HUMSTR. CXCR4 is a G-protein-coupled chemokine receptor that serves as a co-receptor for X4 HIV-1 entry into CD4+ cells and plays a key role in lymphocyte migration. Its ligand is SDF1.

CXCR4 is essential for maintaining granulocyte progenitors in bone marrow and for B cell development. Expression differs between species: in mice, it is restricted to CD4+CD8+ thymocytes, while in humans, expression is predominant in B lymphocytes.

The 2B11 antibody recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1α binding. It is validated for flow cytometric analysis and tested on mouse thymocytes at ≤1 µg/test. Optimal staining requires ~45 minutes of incubation.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a seven-transmembrane G-protein-coupled receptor that functions as a co-receptor for HIV-1 and HIV-2 entry into CD4+ cells. It binds the chemokine CXCL12/SDF-1, leading to calcium signaling and MAPK activation. CXCR4 is involved in hematopoiesis, cardiac and vascular development, and neuronal survival. It also mediates LPS-induced inflammatory responses.

Antibodies against CXCR4 can inhibit HIV-1 and HIV-2 fusion and infection. The amino-terminal domain and second extracellular loop serve as key HIV binding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지