Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), PE, eBioscience
CD184(CXCR4) 단일클론 항체(2B11), PE-conjugated로 마우스 및 인간 시료에서 CXCR4 검출에 사용. Flow cytometry에 최적화되어 있으며 1 µg/test 권장. 0.2 mg/mL 농도의 액상형으로 4°C 암소 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 53 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B11 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891393 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 805
- eFluor 450
- Functional Grade
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Custom conjugation available
Product Specific Information
The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184), also known as Fusin, LESTR, or HUMSTR. CXCR4 is a G-protein-coupled chemokine receptor that serves as a co-receptor for X4 HIV-1 entry into CD4+ cells and plays a key role in lymphocyte migration. Its ligand is SDF1.
CXCR4 is essential for maintaining granulocyte progenitors in bone marrow and for B cell development. Expression differs between species: in mice, it is restricted to CD4+CD8+ thymocytes, while in humans, expression is predominant in B lymphocytes.
The 2B11 antibody recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1α binding. It is validated for flow cytometric analysis and tested on mouse thymocytes at ≤1 µg/test. Optimal staining requires ~45 minutes of incubation.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CXCR4 (Fusin, LESTR, HUMSTR) is a seven-transmembrane G-protein-coupled receptor that functions as a co-receptor for HIV-1 and HIV-2 entry into CD4+ cells. It binds the chemokine CXCL12/SDF-1, leading to calcium signaling and MAPK activation. CXCR4 is involved in hematopoiesis, cardiac and vascular development, and neuronal survival. It also mediates LPS-induced inflammatory responses.
Antibodies against CXCR4 can inhibit HIV-1 and HIV-2 fusion and infection. The amino-terminal domain and second extracellular loop serve as key HIV binding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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