Thermo Fisher Scientific Lambda light chain Monoclonal Antibody (1-155-2), PE, eBioscience
인간 Lambda light chain을 인식하는 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. PE 형광 표지로 488–561 nm 레이저에 적합하며, 5 µL/test로 사용 가능합니다. 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공합니다.
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Thermo Fisher Scientific Lambda light chain Monoclonal Antibody (1-155-2), PE, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution
- 5 µL (0.25 µg)/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1-155-2 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1724133 |
Additional Formats
Product Specific Information
The monoclonal antibody 1-155-2 recognizes the lambda light chain of human immunoglobulin. Approximately 22 kDa, the Ig lambda chain comprises one variable and one constant region. Two lambda chains together with the heavy chain form the complete immunoglobulin structure. Expression of Ig lambda is typically found in less than half of B lymphocytes and has been associated with neoplastic lymphomas.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains and two light chains (kappa, lambda; ~22.5 kDa). Kappa and lambda chains consist of variable and constant regions, differentiated by antigenic properties of the constant region. The kappa-to-lambda ratio is approximately 70:30. Antibodies to the kappa light chain are useful in identifying leukemias, plasmacytomas, and non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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