Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), HRP
ATM 단백질의 Ser1981 인산화 형태를 특이적으로 인식하는 HRP-conjugated 단일클론 항체. Western blot, IHC, ELISA에 적합하며 인간, 마우스, 랫트 시료에 반응. 단백질 A로 정제된 동결건조 형태로 안정적 저장 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:600 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:70,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H11.E12 |
| Immunogen | Synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S (aa 1974–1988 of human ATM) |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | 4°C (short term), -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
Additional Formats
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute immediately before use.
This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and overexpressed proteins found in various tissues and extracts. Reactivity is observed against human and mouse ATM. Cross-reactivity with other mammalian sources has not been tested.
Target Information
Ataxia-telangiectasia Mutated (ATM) is a member of the PI3/PI4 kinase family. The ATM gene encodes a protein involved in DNA double-strand break repair and cell cycle regulation. ATM phosphorylates downstream targets such as P53, Mdm2, BRCA1, and SMC1.
ATM autophosphorylation is induced by ionizing radiation, triggering kinase cascades regulating apoptosis and DNA damage repair. Mutations in ATM cause ataxia-telangiectasia, characterized by neurological degeneration, immunodeficiency, and cancer predisposition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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