Thermo Fisher Scientific MEK2 N-Term Monoclonal Antibody (19G10.F1.E2), HRP
HRP로 결합된 MEK2 N-말단 특이 단클론 항체로, Western blot 및 ELISA에 적합합니다. 인간, 마우스, 랫트에 반응하며 MEK1과는 교차 반응하지 않습니다. 단백질 신호전달 연구용으로 사용됩니다.
- 카탈로그번호
- 200-303-GN8x (2개 옵션)
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 19G10.F1.E2 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the N-terminus of MEK2, conjugated to KLH |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- DyLight 680
- DyLight 800
- FITC
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge if solution is not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
This antibody reacts specifically with human MEK2. Cross-reactivity is expected with mouse and rat based on sequence identity of the peptide immunogen. No reactivity with MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. It activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif of the activation loop. MEK1 and MEK2 are activated by dual phosphorylation at serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, serving as a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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