Thermo Fisher Scientific MEK1 C-Term Monoclonal Antibody (13B6.G12), HRP
HRP로 결합된 MEK1 C-말단 특이 단클론 항체로, Western blot과 ELISA에 적합합니다. 인간, 마우스, 랫트 반응성을 가지며 MEK2와는 반응하지 않습니다. Protein A로 정제되었으며, 장기 보관 시 -20°C에서 보관 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13B6.G12 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- DyLight 680
- DyLight 800
- FITC
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-MEK1 is purified from tissue culture supernatant by Protein A purification. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in MAPK cascades, regulating cell growth and differentiation. It is activated by various growth factors, cytokines, membrane depolarization, and calcium influx. MEK1 activation involves phosphorylation at serine 218 and 222 residues by Raf1. It participates in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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