Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), HRP
DYKDDDDK(FALG) 태그를 인식하는 HRP 결합 단일클론 항체로, Western blot, IHC, ELISA, IP 등 다양한 응용에 적합합니다. 마우스 IgG2a, kappa 아이소타입이며, 높은 특이성과 안정성을 제공하여 단백질 발현 및 정량 분석에 유용합니다.
- 카탈로그번호
- 200-303-383
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), HRP
Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29E4.G7 |
| Immunogen | Synthetic peptide corresponding to FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Glycine and cysteine residues added for coupling. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Agarose
- Biotin
- DyLight 405, 488, 549, 649, 680, 800
- FITC
- Custom conjugation available
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if solution is not clear after standing. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is directed against the FLAG™ epitope tag and is useful for detecting its presence in overexpressed proteins across various assays. It recognizes the FLAG™ tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino or carboxy termini of target proteins in transfected or transformed cells.
Target Information
DYKDDDDK tag (FLAG tag) is an eight amino acid sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) commonly used for protein purification, detection, and localization. It can be fused to the N- or C-terminus of proteins for easy identification using anti-FLAG antibodies. The tag’s hydrophilic nature minimizes protein denaturation or inactivation. Widely used in molecular biology and biochemistry for protein-protein interaction, expression, and localization studies.
FLAG™ is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.