Thermo Fisher Scientific KLF4 Polyclonal Antibody
KLF4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간, 마우스, 설치류 등 다양한 종에 반응. Protein G로 정제된 액상 형태이며 1 mg/mL 농도. 줄기세포 연구 및 세포 분화 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1:10–1:500 | - |
| Flow Cytometry (Flow) | 1 µg per million cells | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A portion of amino acids 1–50 of human KLF4 was used as the immunogen for this antibody. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2540710 |
Target Information
Kruppel-like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. Also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF), it belongs to a conserved family of DNA-binding zinc finger transcription factors.
KLF4 plays a significant role in various cellular pathways and is one of the four Yamanaka factors, essential for reprogramming differentiated cells into pluripotent stem cells. It is involved in processes such as endothelial signaling, adipogenesis, differentiation, embryogenesis, and cell cycle regulation.
The localization of KLF4 determines its biological role: nuclear localization is often associated with oncogenic activity, whereas cytosolic localization can be linked to tumor-suppressive effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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