Thermo Fisher Scientific ATG5 Polyclonal Antibody
ATG5 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 IHC(Paraffin) 분석에 적합합니다. 자가포식 관련 단백질 연구에 유용하며, 고순도의 항원 친화 크로마토그래피 정제 제품입니다. 다양한 종에 반응하며 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A portion of amino acid 1–50 of human APG5L |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2540712 |
Target Information
ATG5 (Autophagy Related 5) is a key component of the autophagy pathway and plays an important role in apoptotic processes. It is involved in mitochondrial quality control after oxidative damage, regulation of innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation.
Following conjugation to ATG12, the ATG5–ATG12 complex participates in autophagosome formation. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5–ATG12 conjugate forms a cup-shaped isolation membrane that detaches before or after autophagosome completion. Additionally, APG5 expression occurs downstream of caspase activity during apoptosis and can interact with immune response proteins such as RIG-I and VISA, modulating interferon production and viral replication. Dysfunction of ATG5 has been associated with spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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