Thermo Fisher Scientific HSP60 Monoclonal Antibody (A57-E4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

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Miscellaneous PubMed (Misc)

-

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Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A57-E4

Immunogen

Recombinant Chlamydia trachomatis HSP60.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325469

Product Specific Information

MA3-022 detects heat shock protein 60 kDa (HSP60) from bacteria. This antibody detects HSP60 from all three species of Chlamydia, Escherichia coli (GroEL), Salmonella typhimurium, Neisseria gonorrhoeae and Coxiella burnetti. MA3-022 does not cross react with HeLa cell HSP60.

MA3-022 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~60 kDa protein representing HSP60 from C. trachomatis lysate.

The MA3-022 immunogen is recombinant Chlamydia trachomatis HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of 6 amino acids of Chlamydia HSP60 amino acids 117-122.

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.