Thermo Fisher Scientific PDI Monoclonal Antibody (RL77)
다종 생물에서 PDI 단백질을 검출하는 RL77 클론 단일클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 비결합형 액상 형태로 안정적이며 -20°C 보관. 연구용으로 단백질 구조 및 기능 분석에 적합.
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Thermo Fisher Scientific PDI Monoclonal Antibody (RL77)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Neutralization (Neu) | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Hamster, Human, Mouse, Pig, Rat, Xenopus |
| Published Species | Hamster, Human, Mouse, Non-human primate, Pig, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RL77 |
| Immunogen | Purified rat PDI protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | PBS, ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2163123 |
Product Specific Information
MA3-018 detects protein disulphide-isomerase (PDI) from human, mouse, rat, porcine, xenopus liver tissues as well as hamster (CHO) cells and canine samples.
It has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures.
By Western blot, this antibody detects a 59 kDa protein representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract.
Immunohistochemical staining of PDI in rat intestine with MA3-018 yields a pattern consistent with cytoplasmic staining.
In immunoprecipitation procedures, MA3-018 has been shown to inhibit the activity of PDI in vitro.
For immunohistochemistry, formalin-fixed paraffin-embedded tissue sections are recommended.
The MA3-018 antigen is purified rat PDI.
Target Information
Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage, and rearrangement of disulfide bonds.
At the cell surface, it acts as a reductase that cleaves disulfide bonds of attached proteins, potentially causing structural modifications of exofacial proteins.
Inside the cell, PDI forms or rearranges disulfide bonds of nascent proteins.
At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins; at low concentrations, it facilitates aggregation (anti-chaperone activity).
PDI may participate with other chaperones in structural modification of the TG precursor in hormone biogenesis.
It also serves as a structural subunit of enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein (MTTP).
It acts as a receptor for LGALS9, retaining P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity, altering plasma membrane redox state, and enhancing cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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