Thermo Fisher Scientific Cyclin H Monoclonal Antibody (33TF2-2D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:400

-

Immunoprecipitation (IP)

1:250-1:500

-

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

33TF2-2D4

Immunogen

Synthetic peptide (KKRKGYEDDDYVSKKSKHEE) corresponding to aa 292-311 (C-terminal region) of human cyclin H. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclin H,uniProtId:P51946-1,ncbiNodeId:9606,antigenRange:292-311,antigenLength:323,antigenImageFileName:MA3-025_Cyclin_H_P51946-1_House_mouse.svg,antigenImageFileNamePDP:MA3-025_Cyclin_H_P51946-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2608589

Product Specific Information

MA3-025 has been used successfully in Western blotting, Immunoprecipitation and Immunofluorescence applications to detect Cyclin H in human samples.

Target Information

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.