Thermo Fisher Scientific Phospho-LIMK1/LIMK2 (Ser323, Ser314) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LIMK1 (Ser-323) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 323 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Ser-314 in human LIMK2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942942

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated LIMK1 (Ser-323) peptide then affinity purified using LIMK1 (Ser-323) peptide (without carrier). The antibody detects a protein with the same mobility as anti-LIMK1 (C-terminus, LP1831) in human A431 cells treated with calyculin A. This band is weak in control cells and is not detected after lambda phosphatase treatment.

Target Information

Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.