Thermo Fisher Scientific Phospho-LIMK1/LIMK2 (Ser323, Ser314) Polyclonal Antibody
Phospho-LIMK1/LIMK2 (Ser323, Ser314) 폴리클로날 항체로 인간, 생쥐, 랫트 반응성. Western blot에 적합하며 세포 이동성과 형태 형성 연구에 활용. 항원 친화 크로마토그래피로 정제된 고품질 항체로 안정적인 액상 형태 제공.
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Applications
- Western Blot (WB): Tested Dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LIMK1 (Ser-323) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 323 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Ser-314 in human LIMK2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942942 |
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated LIMK1 (Ser-323) peptide then affinity purified using LIMK1 (Ser-323) peptide (without carrier). The antibody detects a protein with the same mobility as anti-LIMK1 (C-terminus, LP1831) in human A431 cells treated with calyculin A. This band is weak in control cells and is not detected after lambda phosphatase treatment.
Target Information
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. LIM kinases, including LIMK-1 and LIMK-2, regulate F-Actin stabilization and cofilin activity, playing key roles in cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells by interfering with MAPK-related pathways. Expression patterns of LIMK-1 and LIMK-2 suggest distinct functions during development. A truncated LIMK-2 form found in adult testis may arise from alternative exon initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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