Thermo Fisher Scientific Perforin Polyclonal Antibody
Thermo Fisher Scientific의 Perforin Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하며 WB, IHC, ICC, ELISA 등에 적합합니다. Rabbit IgG 폴리클로날 항체로, 세포막 및 세포질 과립 내 perforin 단백질 검출에 유용합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Perforin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 280–555 of human Perforin (NP_0050322) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.56 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804086 |
Product Specific Information
Immunogen sequence:
EEKKKKHKMT ASFHQTYRER HSEVVGGHHT SINDLLFGIQ AGPEQYSAWV NSLPGSPGLV DYTLEPLHVL LDSQDPRREA LRRALSQYLT DRARWRDCSR PCPPGRQKSP RDPCQCVCHG SAVTTQDCCP RQRGLAQLEV TFIQAWGLWG DWFTATDAYV KLFFGGQELR TSTVWDNNNP IWSVRLDFGD VLLATGGPLR LQVWDQDSGR DDDLLGTCDQ APKSGSHEVR CNLNHGHLKF RYHARCLPHL GGGTCLDYVP QMLLGEPPGN RSGAVW
Positive Samples: Mouse thymus, Mouse kidney
Cellular Location: Cell membrane, Cytoplasmic granule lumen, Endosome lumen, Multi-pass membrane protein
Target Information
Perforin is a major cytolytic protein stored in cytoplasmic granules and plays a critical role in immune defense against tumors and viral infections mediated by cytotoxic lymphocytes. It is a 555 amino acid protein with a 21 amino acid signal peptide and a molecular weight of 70–75 kD. Perforin forms transmembrane channels similar to complement component C9. It is expressed in killer cell lines but not in helper T lymphocytes or other tumor cells. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare autosomal recessive disorder. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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