Thermo Fisher Scientific Prohibitin Polyclonal Antibody
Prohibitin 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, ELISA에 사용 가능. Human, Mouse, Rat 시료 반응성. 미토콘드리아 내막에 위치한 Prohibitin 검출에 적합. 고순도 Affinity 정제 및 안정한 PBS/glycerol buffer 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 73–272 of human Prohibitin (NP_002625.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.36 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804084 |
Product Specific Information
- Positive Samples: Raji, NIH/3T3, HeLa, MCF7, HepG2, Mouse liver, Mouse heart, Mouse kidney, Rat heart
- Cellular Location: Mitochondrion inner membrane
Immunogen sequence:
PRNVPVITGS KDLQNVNITL RILFRPVASQ LPRIFTSIGE DYDERVLPSI TTEILKSVVA RFDAGELITQ RELVSRQVSD DLTERAATFG LILDDVSLTH LTFGKEFTEA VEAKQVAQQE AERARFVVEK AEQQKKAAII SAEGDSKAAE LIANSLATAG DGLIELRKLE AAEDIAYQLS RSRNITYLPA GQSVLLQLPQ
Target Information
Prohibitin is an evolutionarily conserved protein expressed ubiquitously. It is considered a negative regulator of cell proliferation and may act as a tumor suppressor. Mutations in the PHB gene have been associated with sporadic breast cancer.
Two transcript variants with different 3′ untranslated regions exist; the longer transcript is more abundant in proliferating tissues and cells, suggesting a regulatory role.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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